Prostate Specific Antigen anticorps (CF®740)

N° du produit ABIN7659260

L’anticorps Souris Monoclonal anti-Prostate Specific Antigen a été validé pour IHC et IHC (fp). Il convient pour détecter Prostate Specific Antigen dans des échantillons de Humain.

Aperçu rapide pour Prostate Specific Antigen anticorps (CF®740) (ABIN7659260)

Antigène: Prostate Specific Antigen (PSA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prostate Specific Antigen est conjugé à/à la CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: KLK3-801

Détail du produit anti-Prostate Specific Antigen anticorps (CF®740)

Fonction: PSA (Prostate Specific Antigen)(KLK3/801), CF740 conjugate

Attributs du produit: Recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant full-length human KLK3 protein

Isotype: IgG1, kappa

Information d'application

Indications d'application: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Commentaires:

Positive Control: PC12 cells or normal prostate or prostate carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Prostate Specific Antigen

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: PSA

Sujet:

Synonyms: antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

Gene Symbol: KLK3

Tissue Expression: Prostate

Poids moléculaire: 33-34 kDa

ID gène: 354

UniProt: P07288

Pathways: Système du Complément